By Donald A Wellings
A distillation of over 20 years of the author's useful adventure of this method, this booklet intentionally steers away from complicated theoretical points and concentrates extra at the 'black paintings' components of the suggestions concerned. accordingly is a e-book for common use within the laboratory, particularly of curiosity to these within the pharmaceutical, agrochemical and biotechnology industries and to a person all for the research and purification of substances, small molecules, proteins and DNA.
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Extra resources for A Practical Handbook of Preparative HPLC
The friction caused by the eluent passing over stationary phase particles generates heat, which in turn reduces the viscosity of the solvent. Cooling by conduction in the vicinity of the column walls reduces the viscosity of the solvent close to the wall in comparison to that at the centre of the column. Consequently, the solvent at the centre of the column is now travelling at a Fluid Dynamics, Mass Transport and Friction 27 higher flow rate than that at the column walls, resulting in a parabolic flow profile, and subsequently, to band broadening.
Ion exchange chromatography, as the name suggests, separates molecules by taking advantage of a charge differential. There are understandably two approaches to ion exchange separations, so both cation and anion exchange stationary phases are commercially available. The stationary phases are predominantly polymer based and functionalized with acidic groups to produce cation exchange materials, or basic groups to produce anion exchangers. Cation exchange media are generally of carboxylic acid or sulfonic acid functionality to provide weak and strong cation exchangers, respectively.
3. 3 For purpose of illustration, the green line represents the results of a typical van Deemter plot. In Zone 1 the low flow rate allows extensive longitudinal diffusion, which ultimately will result in diffusion against the direction of flow. At high flow rates shown in Zone 2, the decreased efficiency is a result of comparatively slow mass transfer. The blue line represents a situation where mass transport is relatively efficient. This might be observed when particle size is small, pore structure is large and the molecular dimensions of the analyte are small.