Antibiotics by John H. Hash

By John H. Hash

The significantly acclaimed laboratory regular, tools in Enzymology, is without doubt one of the so much hugely revered courses within the box of biochemistry. because 1955, every one quantity has been eagerly awaited, usually consulted, and praised through researchers and reviewers alike. The sequence includes a lot fabric nonetheless appropriate at the present time - really a necessary book for researchers in all fields of lifestyles sciences.

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B. T r a n s d u c t i o n ~ Transduction of resistance plasmids was observed soon after they were discovered. Bacteriophage P1 kc has been used with E. coli, and this phage transduces all the resistances plus the resistance transfer factor of a conjugative resistance plasmid. On the other hand, bacteriophage P22, which is used with S. t y p h i m u r i u m is not capable of transducing the entire resistance plasmid and transduction by P22 often leads to loss of transferability and loss of one or more of the resistance characters.

C. Transformation . . . . . . . III. Enhanced Segregation of Resistance Plasmids by Curing Agents A. Acridine Orange, Quinacrine, and Ethidium Bromide Curing B. Sodium Dodecyl Sulfate . . . . . . C. Mitomycin . . . . . . . . IV. Compatibility Properties of R Plasmids . . . . A. Determination of fi Character . . . . . B. Testing for Compatibility of R Factors . . . . . . . . . . . . . . 41 43 43 45 48 49 51 51 52 52 54 54 I. I n t r o d u c t i o n T h e extensive use of c h e m o t h e r a p e u t i c agents has p r o v i d e d a n e n v i r o n m e n t conducive to the selection of b a c t e r i a l s t r a i n s I r e s i s t a n t to these agents.

In crosses involving P. aeruginosa and E. coli, since P. aeruginosa is a strict aerobe, anaerobic conditions (anaerobic jar) can be used to counterselect against the P. aeruginosa strain although this is not as convenient as counterselection with an antimicrobial agent, such as rifampicin. I t is important to realize that failure to observe transfer does not mean that a resistance plasmid is not present and even less so that the resistance genes in question are chromosomal. I t is often necessary to examine a large number of bacterial isolates for the presence of con]ugative plasmids, and under these circumstances it is unwieldy to set up many tube crosses in liquid medium, as described above.

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